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Substrate solution is then added and color develops in proportion to the amount of Mouse ICOSLG
Then Streptavidin-HRP is added and binds to the Biotinylated ANXA3 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OxLDL
The concentration of Rat LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Rat NPPB
Rat MDK (Midkine) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat (MDK) Midkine ELISA Kit measures Midkine in Rat samples. The plate has been pre coated with Rat MDK antibody. MDK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MDK Antibody is added and binds to MDK in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MDK antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution is
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