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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LIPB
Only those wells that contain Mouse D-LDH
This peptide possesses nanomolar-affinity for streptavidin and therefore is termed Nano-tag
competition occurs between angiotensin-I present in calibrators
Substrate solution is then added and color develops in proportion to the amount of Human APOC2
Mouse IRS-1 (Insulin Receptor Substrate 1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Mouse (IRS 1) Insulin Receptor Substrate 1 ELISA Kit measures Insulin Receptor Substrate 1 in Mouse samples. The plate has been pre coated with Mouse IRS1 antibody. IRS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IRS1 Antibody is added and binds to IRS1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IRS1 antibody. After incubation unbound Streptavidin HRP is