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Mouse IgG2c in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG2c
Streptavidin-HRP conjugate is added into the well
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GFER
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TUBb
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGF21
Human IL1F5 (Interleukin-36 Receptor Antagonist Protein) ELISA Kit Packaging:96 wells Mouse IgG2c in samples (orThe Human (IL1F5) Interleukin 36 Receptor Antagonist Protein ELISA Kit measures Interleukin 36 receptor antagonist protein in samples. The plate has been pre coated with Human IL1F5 antibody. IL1F5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL1F5 Antibody is added and binds to IL1F5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL1F5 antibody. After incubation
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