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SEMA4C present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Human KLF5 antibody
but their precise roles are yet to be described
Then Streptavidin-HRP is added and binds to the Biotinylated MUC12 antibody
The Rabbit GPR37 (G Protein Coupled Receptor 37) ELISA immunoassay kit uses the Sandwich-ELISA principle
Rat IL-1Alpha (Interleukin 1 Alpha) ELISA Kit Packaging:48 wells SEMA4C present in the sampleThe Rat IL 1Alpha (Interleukin 1 Alpha) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat IL 1Alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL 1Alpha and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each
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