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Then a biotinylated detection antibody specific for Human IL-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of A1BG in the sample by plotting standard curve
SFRP5 present in the sample is added and binds to antibodies coated on the wells
The concentration of Human VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MyD88
Human AMFR (E3 ubiquitin-protein ligase AMFR) QuickTest ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human AMFR (E3 ubiquitin protein ligase AMFR) QuickTest ELISA Kit measures AMFR in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot
