Human AP (Allopregnanolone) ELISA Kit Packaging:96 wells only those wells that contain

only those wells that contain Human PDGFA

Bovine Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol

Then Streptavidin-HRP is added and binds to the Biotinylated CLSPN antibody

Then a biotinylated detection antibody specific for Rat hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

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Human AP (Allopregnanolone) ELISA Kit Packaging:96 wells only those wells that containThe Human AP (Allopregnanolone) ELISA Kit measures AP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with AP. AP in the sample or standard competes with a fixed amount of biotin labeled AP for sites on a pre coated antibody specific to AP. Free components are washed off. After adding HRP