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and the color intensity is directly proportional to the amount of Human CD300C present
Calculate the concentration of Adcyap1 in the sample by plotting standard curve
PCX present in the sample is added and binds to antibodies coated on the wells
The color development is stopped with the addition of 50uL stop solution
Calculate the concentration of ANLN in the sample by plotting standard curve
Canine OPN (Osteopontin) ELISA Kit Packaging:96 wells and the color intensity isThe Canine OPN (Osteopontin) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Canine OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Canine OPN and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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