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Substrate solution is then added and color develops in proportion to the amount of Human AKR1C3
POTEE present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated SYT15 antibody
it binds with p-Akt (Ser473) bound to precoated antibody
The plate has been pre-coated with Mouse BMI1 antibody
Human FAIM3 (Fas Apoptotic Inhibitory Molecule 3) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human FAIM3 (Fas Apoptotic Inhibitory Molecule 3) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human FAIM3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FAIM3 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively
