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METRNL present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Mouse IL17RA
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PⅢCP
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ECF
Then a biotinylated detection antibody specific for Human TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat UCN (Urocortin) ELISA KIT TGFA METRNL present in the sampleThe Rat (UCN) Urocortin ELISA KIT measures Urocortin in Rat samples. The plate has been pre coated with Rat UCN antibody. UCN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat UCN Antibody is added and binds to UCN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated UCN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
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