Human TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit FHC Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Rat SHH

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFa3

The OD value is proportional to the concentration of Human POSTN/OSF-2

FBLN5 present in the sample is added and binds to antibodies coated on the wells

The plate has been pre-coated with Human IMPDH2 antibody

Human TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit FHC Substrate solution is then addedThe Human TNF Alpha (Tumor Necrosis Factor Alpha) ELISA Kit measures TNF Alpha in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add