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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HDL
Then Streptavidin-HRP is added and binds to the Biotinylated Shank3 antibody
The concentration of Sheep IL13 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Mouse EFEMP1 Antibody is added and binds to EFEMP1 in the sample
Substrate solution is then added and color develops in proportion to the amount of Human NR4A2
Human NEU3 (Sialidase-3) ELISA Kit Packaging:96 wells The micro ELISA plate providedThe Human (NEU3) Sialidase 3 ELISA Kit measures Sialidase 3 in samples. The plate has been pre coated with Human NEU3 antibody. NEU3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NEU3 Antibody is added and binds to NEU3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NEU3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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