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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 31 nm
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASGR2
Universal ADMA in the sample or standard competes with a fixed amount of Universal ADMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ADMA
The assay plate has been pre-coated with mouse anti-Human IL-7 monoclonal antibody
Human ALB in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human ALB
Canine HCRT (Orexin) ELISA Kit CD144 The optical density (OD) isThe Canine (HCRT) Orexin ELISA Kit measures Orexin in Canine samples. The plate has been pre coated with Canine HCRT antibody. HCRT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine HCRT Antibody is added and binds to HCRT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HCRT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
