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Substrate solution is then added and color develops in proportion to the amount of Bovine TRAF6
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLEC7A
The concentration of Human MSTN in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human FGF21 in the samples is then determined by comparing the OD of the samples to the standard curve
Histone H2A (Acetyl Lys5) Rabbit Polyclonal Antibody
Rat LR (Leptin Receptor) ELISA Kit TNFSF20 Substrate solution is then addedThe Rat (LR) Leptin Receptor ELISA Kit measures Leptin Receptor in Rat samples. The plate has been pre coated with Rat LEPR antibody. LEPR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LEPR Antibody is added and binds to LEPR in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LEPR antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
