Goat/Sheep PTMA (Prothymosin alpha) ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human COL3α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

And then biotinylated Rabbit PIGR Antibody is added and binds to PIGR in the sample

The concentration of Rabbit IL12 in the samples is then determined by comparing the OD of the samples to the standard curve

FZD4 present in the sample is added and binds to antibodies coated on the wells

Substrate solution is then added and color develops in proportion to the amount of Human SMAD6

Goat/Sheep PTMA (Prothymosin alpha) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Goat Sheep PTMA (Prothymosin alpha) ELISA Kit measures PTMA in Goat Sheep Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PTMA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated