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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish TNFa
Substrate solution is then added and color develops in proportion to the amount of Human FAM13A
The concentration of Rat NFE2L2 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated PLA2G7 antibody
The plate has been pre-coated with Human DEGS2 antibody
Human TMEM27 (Collectrin) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (TMEM27) Collectrin ELISA Kit measures Collectrin in samples.
