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Substrate solution is then added and color develops in proportion to the amount of Mouse EDN3
Then Streptavidin-HRP is added and binds to the Biotinylated CA9 antibody
TNFRSF17 present in the sample is added and binds to antibodies coated on the wells
Calculate the concentration of Cortactin in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated ATP2C1 antibody
Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat BMP 2 (Bone Morphogenetic Protein 2) ELISA Kit measures BMP 2 in Rat Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BMP 2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with BMP 2
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