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the intensity of color is proportional to the concentration of hGH in the samples
The OD value is proportional to the concentration of Rat OPN
You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse IFN-gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Rat TNFSF4 ELISA kit is a sandwich enzyme immunoassay
aFP (Alpha-Fetoprotein) ELISA Kit (Small Sample) Packaging:96 wells the intensity of color isThe aFP (Alpha Fetoprotein) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human aFP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human aFP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
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