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Human DKK1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human DKK1
The concentration of Rat IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human IgG in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA kit uses the Sandwich-ELISA principle
MMP10 (Matrix Metalloproteinase 10) ELISA Kit (Small Sample) Packaging:48 wells Human DKK1 in samples (orThe MMP10 (Matrix Metalloproteinase 10) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human MMP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human MMP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
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