Then Streptavidin-HRP is added and binds to the Biotinylated IL-1A antibody
And then biotinylated Human OTOL1 Antibody is added and binds to OTOL1 in the sample
You can calculate the concentration of Human IL-27 in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human Pâ…¡CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL18
Human PTEN (Phosphatidylinositol-3,4, 5-Trisphosphate 3-phosphatase and Dual-specificity Protein Phosphatase Pten) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human (PTEN) Phosphatidylinositol 3,4, 5 Trisphosphate 3 phosphatase and Dual specificity Protein Phosphatase Pten ELISA Kit measures Phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase and dual specificity protein phosphatase PTEN in samples. The plate has been pre coated with Human PTEN antibody. PTEN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PTEN Antibody is added and binds to