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Then a biotinylated detection antibody specific for Mouse IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated PKP4 antibody
The plate has been pre-coated with Mouse F7 antibody
Calculate the concentration of HMGN1 in the sample by plotting standard curve
And then biotinylated Human EIF2C2 Antibody is added and binds to EIF2C2 in the sample
Human IRF1 (Interferon regulatory factor 1) QuickTest ELISA Kit Hepapoietin Then a biotinylated detection antibodyThe Human IRF1 (Interferon regulatory factor 1) QuickTest ELISA Kit measures IRF1 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot
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