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The concentration of Rat IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Mouse F8
only those wells that contain Human 5HTR2C
And then biotinylated Human CYTH2 Antibody is added and binds to CYTH2 in the sample
Human GUCA2A (Guanylin) ELISA Kit Packaging:96 wells The concentration of Rat IL-2sRa/CD25The Human (GUCA2A) Guanylin ELISA Kit measures Guanylin in samples. The plate has been pre coated with Human GUCA2A antibody. GUCA2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GUCA2A Antibody is added and binds to GUCA2A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GUCA2A antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
